SubtiBank SubtiBank
accB [2017-06-09 08:55:00]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

accB [2017-06-09 08:55:00]

acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
locus
BSU24350
pI
4.39
mw
17.09 kDa
protein length
159 aa Sequence Blast
gene length
477 bp Sequence Blast
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
essential
yes
ec
6.4.1.2
synonyms
fabE, yqhW
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    2,531,718 → 2,532,197

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    [SW|Cofactors]

  • biotin [Pubmed|24816803]

    • Structure

  • [PDB|1BDO] (the protein from ''E. coli'') [Pubmed|8747466]

    • Additional information

  • AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [SW|SPINE].

    • Expression and Regulation

    Operons

    genes
    [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]
    description
    [Pubmed|7592499]

    regulation

  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]
    • view in new tab

    Biological materials

    Expression vector

  • GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP1027: IPTG inducible expression, purification in ''E. coli'' without any tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
  • pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
  • pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1493 (mls, based on [SW|pGP1080]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1484 (''mls'', based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 15952903,12121720,15968460

    • Original Publications

  • 7592499,8747466,24816803