secA [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel

locus: BSU35300

pI: 5.00

mw: 95.00 kDa

function: protein secretion

product: preprotein translocase subunit (ATPase)

essential: yes

synonyms: div, div-341, ts-341

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|protein secretion], [SW|essential genes], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence): 3,628,310 -> 3,630,835

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP -> ADP Pi preprotein translocation
• required for membrane targeting of [SW|DivIVA] [Pubmed|24592260]

Protein family

• SecA family (according to Swiss-Prot)

Paralogous protein(s)

• none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range

[SW|Domains]

• nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain

[SW|Cofactors]

• Mg

Effectors of protein activity

• anionic phospholipids, preprotein, [protein|search|SecY], signal peptides (even when added in trans) [Pubmed|19924216]

Structure

• [PDB|1TF5] (open structure), [PDB|2IBM], [PDB|3DL8] (structure of the ([protein|search|SecY]-[protein|search|SecE]-[protein|search|SecG])-[protein|search|SecA] complex [Pubmed|18923516]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

[SW|Interactions]

• ([protein|search|SecY]-[protein|search|SecE]-[protein|search|SecG])2-[protein|search|SecA]2 [Pubmed|18923516]
• [protein|search|SecA]-[protein|search|Ffh] [Pubmed|9880811]
• [protein|search|CsaA]-[protein|search|SecA] [Pubmed|10816431]
• [SW|DivIVA]-[protein|search|SecA] [Pubmed|24592260]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operon

• ''[protein|search|secA]-[protein|search|prfB]'' [Pubmed|9882663]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|9882663]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

References

Reviews

• 25212246

Original publications

• 19933328,19924216,18923516,12873133,12242434,1385592,10816431,19850053,9880811,12218047,17981983,9882663,23484952,20574771,23167435,23794293,23852076,24592260,24786965,11021932,7851746,7894702,10074074,8497195,8440733,7706219,7642557,27336478