secA

preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel

Locus: BSU_35300

Isoelectric point: 5.34

Molecular weight: 95.33 kDa

Protein length: 841 aa

Gene length: 2526 bp

Function: protein secretion

Product: preprotein translocase subunit (ATPase)

Essential: yes

Synonyms: div,div-341,ts-341

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein secretion
• Groups of genes → Essential genes
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,628,310 3,630,835

Phenotypes of a mutant

• essential PubMed
• terminal phenotype of CRISPRi knockdown mutant is moderate bending and a cessation of growth PubMed

The protein

Catalyzed reaction/ biological activity

• ATP ADP + Pi + preprotein translocation
• required for membrane targeting of DivIVA PubMed

Protein family

• SecA family (single member, according to UniProt)

Domains

• nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain

Cofactors

• Mg

Effectors of protein activity

• anionic phospholipids, preprotein, SecY, signal peptides (even when added in trans) PubMed

Structure

• 1TF5 (open structure), 2IBM, 3DL8 (structure of the (SecY-SecE-SecG)-SecA complex PubMed

Localization

• cell membrane (according to UniProt)
• cytoplasm (according to UniProt)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: secA-prfB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• BEC35300, a CRISPRi knockdown mutant, is available from the Bacillus Genetic Stock Center

References

Reviews

Loading

Original publications

Loading