mfd [Fri Aug 19 2016 15:42:10 GMT+0200 (CEST)]

transcription-repair coupling factor, eliminates genetic damage from transcriptionally active genes during sporulation, required for increased mutagenesis of lagging strand genes

locus: BSU00550

pI: 5.00

mw: 133.00 kDa

function: promotes strand-specific DNA repair by displacing

product: transcription-repair coupling factor

essential: no

ec: 3.6.4.-

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.3|Transcription elongation/ termination]

[SW|Categories] containing this gene/protein

• [SW|DNA repair/ recombination], [SW|transcription]

Gene

Coordinates on the chromosome (coding sequence): 60,430 -> 63,963

Phenotypes of a mutant

• in an ''mfd'' knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed [http://www.pubmed.com/16950921 PubMed]
• strongly reduced stationary phase mutagenesis [Pubmed|27399782]
• increased UV-induced mutagenesis via [SW|PolY1]/ [SW|PolY2]-mediated translesion synthesis [Pubmed|24118570]
• the mutation suppresses the mucoid phenotype of ''[protein|search|motA]'' or ''[protein|search|motB]'' mutants [Pubmed|24296669]

The protein

Catalyzed reaction/ biological activity

• promotes strand-specific DNA repair by displacing [SW|RNA polymerase] stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site
• is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for [protein|search|CcpA] [Pubmed|9535092] and [protein|search|CodY] [Pubmed|21699902])
• required for the processing of genetic damage during [SW|sporulation] [Pubmed|24118570]

Paralogous protein(s)

• [protein|search|RecG]

Structure

• [http://www.proteopedia.org/wiki/index.php/2eyq 2EYQ] (Mfd from ''E. coli'') [Pubmed|16469698]
• [PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|search|Mfd] with the ''Thermus aquaticus'' [protein|search|RpoB] beta1 domain) [Pubmed|20702425]

[SW|Interactions]

• [protein|search|Mfd] interacts with the [SW|RNA polymerase] [Pubmed|21710567]
• [protein|search|Mfd]-[protein|search|UvrA] [Pubmed|16464004]
• [protein|search|Mfd]-[protein|search|RpoB] [Pubmed|20702425]

Expression and Regulation

Regulation

• expressed throughout growth and [SW|sporulation], during [SW|sporulation] both in the mother cell and the forespore [Pubmed|24118570]

Biological materials

Mutant

• GP1167 (del ermC), available in [SW|Jörg Stülke]'s lab
• BKE00550 (''[protein|search|mfd]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s labs)

GFP fusion

• GP1510 (spc, based on [SW|pGP1870], [SW|pGP1389]-derivative ), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

Antibody

• **

References

Reviews

• 17239578,15063847,17917874,8811173,7968917,15720542,16464004,19023283,22201950,22749141,22933559,26132790

Original publications

• 8594198,20435731,104170,16950921,9535092,16469698,21699902,22178973,22248542,22960746,21710567,20702425,24296669,21145481,24118570,25326311,25961799,25713353,27399782