mfd [2017-07-24 14:22:29]

transcription-repair coupling factor, eliminates genetic damage from transcriptionally active genes during sporulation, required for increased mutagenesis of lagging strand genes

locus: BSU00550

pI: 5.37

mw: 133.59 kDa

protein length: 1177 aa

gene length: 3531 bp

function: promotes strand-specific DNA repair by displacing

product: transcription-repair coupling factor

essential: no

ec: 3.6.4.-

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.3|Transcription elongation/ termination]

Gene

Coordinates: 60,430 → 63,963

Phenotypes of a mutant

• in an ''mfd'' knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed [http://www.pubmed.com/16950921 PubMed]
• strongly reduced stationary phase mutagenesis [Pubmed|27399782]
• increased UV-induced mutagenesis via [protein|C87D33852F263A553CA747608E6200E4AD8344B5|PolY1]/ [protein|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|PolY2]-mediated translesion synthesis [Pubmed|24118570]
• the mutation suppresses the mucoid phenotype of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]'' or ''[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' mutants [Pubmed|24296669]

The protein

Catalyzed reaction/ biological activity

• promotes strand-specific DNA repair by displacing [SW|RNA polymerase] stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site
• is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA] [Pubmed|9535092] and [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY] [Pubmed|21699902])
• required for the processing of genetic damage during [SW|sporulation] [Pubmed|24118570]

Paralogous protein(s)

• [protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|RecU]

Structure

• [PDB|2EYQ] (Mfd from ''E. coli'') [Pubmed|16469698]
• [PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|AD1F39823ABCE2A222259B879513752942C59590|Mfd] with the ''Thermus aquaticus'' [protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|RpoB] beta1 domain) [Pubmed|20702425]

Expression and Regulation

Operons

• genes: [gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]-[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]-[gene|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]
  description: [pubmed|22383849]

Biological materials

Mutant

• GP1167 (del ermC), available in [SW|Jörg Stülke]'s lab [Pubmed|22178973]
• BKE00550 (''[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s labs) [pubmed|28189581]

GFP fusion

• GP1510 (spc, based on [SW|pGP1870], [SW|pGP1389]-derivative ), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

Antibody

• **

References

Reviews

• 17239578,15063847,17917874,8811173,7968917,15720542,16464004,19023283,22201950,22749141,22933559,26132790

Original publications

• 8594198,20435731,104170,16950921,9535092,16469698,21699902,22178973,22248542,22960746,21710567,20702425,24296669,21145481,24118570,25326311,25961799,25713353,27399782,28700593