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purM [Mon Aug 31 2015 13:52:56 GMT+0200 (CEST)]
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purM [Mon Aug 31 2015 13:52:56 GMT+0200 (CEST)]

phosphoribosylaminoimidazole synthetase
locus
BSU06500
pI
4.59
mw
36.00 kDa
function
purine biosynthesis
product
phosphoribosylaminoimidazole synthetase
essential
no
ec
6.3.3.1
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of nucleotides]

    • This gene is a member of the following [SW|regulons]

  • [SW|G-box], [SW|PurR regulon]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole (according to Swiss-Prot)

    • Protein family

  • AIR synthase family (according to Swiss-Prot)

    • Structure

  • [PDB|2BTU] (from ''Bacillus anthracis'', 64% identity, 78% similarity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operon

  • ''[protein|search|purE]-[protein|search|purK]-[protein|search|purB]-[protein|search|purC]-[protein|search|purS]-[protein|search|purQ]-[protein|search|purL]-[protein|search|purF]-[protein|search|purM]-[protein|search|purN]-[protein|search|purH]-[protein|search|purD]'' [Pubmed|3036807]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|3036807]

    • Regulation

  • repressed in the presence of purine nucleotides ([protein|search|PurR]) [Pubmed|7638212]
  • repressed in the presence of adenine or adenosine([protein|search|PurR]) [Pubmed|2536750]
  • repressed in the presence of guanine([protein|search|G-box]) [Pubmed|3036807]

    • Regulatory mechanism

  • [PurR]]: transcription repression [Pubmed|7638212]
  • [protein|search|PurR]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]
  • [protein|search|G-box]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]

    • Biological materials

    Mutant

  • 1A601 ( ''purM''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A601&Search=1A601 BGSC]

    • References

  • 3036807,12923093,7638212