purM [2019-06-24 08:42:59]

phosphoribosylaminoimidazole synthetase

locus: BSU06500

pI: 4.59

mw: 36.90 kDa

protein length: 346 aa

gene length: 1041 bp

function: purine biosynthesis

product: phosphoribosylaminoimidazole synthetase

essential: no

ec: 6.3.3.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]

Gene

Coordinates: 706,973 708,013

The protein

Catalyzed reaction/ biological activity

• ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole (according to Swiss-Prot)

Protein family

• AIR synthase family (according to Swiss-Prot)

Structure

• [PDB|2BTU] (from ''Bacillus anthracis'', 64% identity, 78% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]-[gene|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|purK]-[gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB]-[gene|447D12E940C7F45B6C5015844F9DED9D513CD6E5|purC]-[gene|CCD4DA969086181CFA33D6D241F2CAAE02E0C758|purS]-[gene|DEE856E83E790EB57F103F7A9156408AF090995D|purQ]-[gene|D34203FAA84C19B34B3CB5F00AC2E2663FFA344D|purL]-[gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]-[gene|AD0DE9457D70DF02FA0F58867B24ABF236FAF65A|purM]-[gene|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]-[gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]-[gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]
  description: [Pubmed|3036807]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3036807], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|G-box|G-box]: termination, ([SW|riboswitch]) [pubmed|3036807,12787499], in [regulon|G-box|G-box]
  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, (molecular inducer: PRPP) [Pubmed|7638212,2536750], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]

  regulation

  • expression activated by glucose (4.4 fold) [Pubmed|12850135]

Biological materials

Mutant

• 1A601 ( ''purM''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A601&Search=1A601 BGSC]
• BKE06500 ([gene|AD0DE9457D70DF02FA0F58867B24ABF236FAF65A|purM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTATTCACTCCTTTAA, downstream forward: _UP4_ACATTCGGCGGTGCGGCACT
• BKK06500 ([gene|AD0DE9457D70DF02FA0F58867B24ABF236FAF65A|purM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTATTCACTCCTTTAA, downstream forward: _UP4_ACATTCGGCGGTGCGGCACT

References

• 3036807,12923093,7638212