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leuC [2018-01-03 11:44:14]
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leuC [2018-01-03 11:44:14]

3-isopropylmalate dehydratase (large subunit)
Locus
BSU28260
Isoelectric point
6.13
Molecular weight
52.23 kDa
Protein length
472 aa Sequence Blast
Gene length
1416 bp Sequence Blast
Function
biosynthesis of leucine
Product
3-isopropylmalate dehydratase (large subunit)
Essential
no
E.C.
4.2.1.33
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of branched-chain amino acids
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    2,889,552 → 2,890,970

    The protein

    Catalyzed reaction/ biological activity

  • (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H2O (according to Swiss-Prot)

    • Protein family

  • LeuC type 1 subfamily (according to Swiss-Prot)

    • Modification

  • phosphorylated on Arg-81 PubMed

    • Cofactors

  • contains an iron-sulfur cluster

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • T-box: termination/antitermination, via tRNA controlled RNA switch, repression by BCAA, in T-box
  • TnrA: repression, PubMed, in TnrA regulon
  • CodY: repression, PubMed PubMed, in CodY regulon
  • CcpA: activation, PubMed, in CcpA regulon

    • Regulation

  • expression is stimulated in the presence of glucose (CcpA) PubMed

    • Additional information

  • An antisense RNA is predicted for leuA PubMed
  • the T-box RNA is degraded by RNase Y PubMed
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    Other regulations

  • FsrA: translation repression, PubMed

    • Biological materials

    Mutant

  • BKE28260 (ΔleuC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTATTTCCTCCCTC, downstream forward: _UP4_ACAGTTGTGTAAGGAGTGCG
  • BKK28260 (ΔleuC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTATTTCCTCCCTC, downstream forward: _UP4_ACAGTTGTGTAAGGAGTGCG

    • References

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