leuC

3-isopropylmalate dehydratase (large subunit)

Locus: BSU_28260

Isoelectric point: 6.13

Molecular weight: 52.23 kDa

Protein length: 472 aa

Gene length: 1419 bp

Function: biosynthesis of leucine

Product: 3-isopropylmalate dehydratase (large subunit)

Essential: no

E.C.: 4.2.1.33

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of branched-chain amino acids
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 2,889,552 2,890,970

The protein

Catalyzed reaction/ biological activity

• (2R,3S)-3-isopropylmalate --> (2S)-2-isopropylmalate (according to UniProt)

Protein family

• aconitase/IPM isomerase family (with CitB, according to UniProt)

Modification

• phosphorylated on Arg-81 PubMed

Cofactors

• Fe-S cluster PubMed

Structure

• 4KP2 (from Methanococcus jannaschii, 35% identity)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • T-box: termination/antitermination, via tRNA controlled RNA switch, repression by BCAA, in T-box
  • TnrA: repression, PubMed, in TnrA regulon
  • CodY: repression, PubMed PubMed, in CodY regulon
  • CcpA: activation, PubMed, in CcpA regulon

  Regulation

  • expression is stimulated in the presence of glucose (CcpA) PubMed

  Additional information

  • An antisense RNA is predicted for leuA PubMed
  • the T-box RNA is degraded by RNase Y PubMed

Other regulations

• FsrA: translation repression, PubMed

Biological materials

Mutant

• BKE28260 (leuC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTATTTCCTCCCTC, downstream forward: _UP4_ACAGTTGTGTAAGGAGTGCG
• BKK28260 (leuC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTATTTCCTCCCTC, downstream forward: _UP4_ACAGTTGTGTAAGGAGTGCG

References

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