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rbsA [2019-04-24 13:20:47]
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rbsA [2019-04-24 13:20:47]

ribose ABC transporter (ATP-binding protein)
Locus
BSU35940
Isoelectric point
6.18
Molecular weight
54.36 kDa
Protein length
493 aa Sequence Blast
Gene length
1482 bp Sequence Blast
Function
ribose uptake
Product
ribose ABC transporter (ATP-binding protein)
Essential
no
E.C.
3.6.3.17
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → ABC transporters → Importers → Uptake of carbon sources
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of ribose
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,703,682 3,705,163

    The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + monosaccharide(Out) = ADP + phosphate + monosaccharide(In) (according to Swiss-Prot)

    • Paralogous protein(s)

  • NupO

    • Structure

  • 1G9X (from Methanocaldococcus jannaschii, 36% identity) PubMed

    • Localization

  • attached to the cell membrane (via RbsC-RbsD) PubMed

    • Expression and Regulation

    Operons

    Genes
    rbsR-rbsK-rbsD-rbsA-rbsC-rbsB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AbrB: activation, PubMed, in AbrB regulon

    • Regulation

  • expressed upon transition into the stationary phase (AbrB) PubMed
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    Biological materials

    Mutant

  • BKE35940 (rbsA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCTGCATGTTTCATCTTC, downstream forward: _UP4_ACACTTGCCACGGGAGGGCG
  • BKK35940 (rbsA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCTGCATGTTTCATCTTC, downstream forward: _UP4_ACACTTGCCACGGGAGGGCG

    • References

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