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guaB [Tue Feb 14 2017 11:41:55 GMT+0100 (CET)]
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guaB [Tue Feb 14 2017 11:41:55 GMT+0100 (CET)]

IMP dehydrogenase
locus
BSU00090
pI
6.00
mw
52.00 kDa
function
biosynthesis of GMP
product
IMP dehydrogenase
essential
no
ec
1.1.1.205
synonyms
guaA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of nucleotides], [SW|essential genes], [SW|phosphoproteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CodY regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    15,915 -> 17,381

    Phenotypes of a mutant

  • essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
  • reduced expression of ''[protein|search|guaB]'' suppresses the requirement of a ''[protein|search|relA] [protein|search|sasA] [protein|search|sasB]'' triple mutant for branched chain amino acids, methionine and threonine [Pubmed|24163341]

    • The protein

    Catalyzed reaction/ biological activity

  • Inosine 5'-phosphate NAD H2O = xanthosine 5'-phosphate NADH (according to Swiss-Prot)

    • Protein family

  • IMPDH/GMPR family (according to Swiss-Prot)

    • [SW|Domains]

  • 2 [SW|CBS domain]s (95-153, 157-214)

    • Modification

  • phosphorylated (STY) [Pubmed|17726680]
  • Cys308 is S-cysteinylated after diamide stress [Pubmed|17611193], [Pubmed|17726680]
  • Cys308 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|22938038]

    • Effectors of protein activity

  • inhibition of enzymatic activity by (p)ppGpp during the 'stringent response'[Pubmed|22981860,6111556]

    • Structure

  • [PDB|1VRD] (from ''Thermotoga maritima msb8'', 60% identity, 80% similarity)

    • Expression and Regulation

    Operon

  • ''guaB'' [Pubmed|22383849]

    • Regulation

  • activated during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]

    • Regulatory mechanism

  • [protein|search|CodY]: transcription activation [Pubmed|12618455]

    • Additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
  • inhibition of enzymatic activity by (p)ppGpp during the 'stringent response' [PubMed|6111556]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • the mRNA is cleaved by [rnc|search|RNase III] [PubMed|26883633]

    • Biological materials

    Expression vector

  • purification from ''B. subtilis'' with an N-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP380]): pGP901, available in [SW|Jörg Stülke]'s lab
  • pGP2937 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 22938038,17611193,12884008,1722815,22981860,12618455,17726680,17726680,6111556,24163341,15378759,24682323,25572472,26883633,28189581