iolC

formation of 2-deoxy-5-keto-gluconic acid-6-phosphate (5th reaction)

Locus: BSU_39740

Isoelectric point: 4.78

Molecular weight: 35.48 kDa

Protein length: 325 aa

Gene length: 978 bp

Function: myo-inositol catabolism

Product: formation of 2-deoxy-5-keto-gluconic acid-6-phosphate (5th reaction)

Essential: no

E.C.: 2.7.1.92

Synonyms: yxdC

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of inositol

Gene

Coordinates: 4,081,029 4,082,006

The protein

Catalyzed reaction/ biological activity

• 5-dehydro-2-deoxy-D-gluconate + ATP --> 6-phospho-5-dehydro-2-deoxy-D-gluconate + ADP + H⁺ (according to UniProt)

Protein family

• carbohydrate kinase PfkB family (according to UniProt)

Structure

• 2QCV (from Bacillus halodurans c-125 mutant, 73% identity, 85% similarity)

Expression and Regulation

Operons

• Genes: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • IolR: repression, PubMed, in IolR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced by inositol (IolR) PubMed

Biological materials

Mutant

• MGNA-B772 (iolC::erm), available at the NBRP B. subtilis, Japan
• BKE39740 (iolC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTAAACAGCCACTCCT, downstream forward: _UP4_TAATTGAAAAAGATCGAGTA
• BKK39740 (iolC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTAAACAGCCACTCCT, downstream forward: _UP4_TAATTGAAAAAGATCGAGTA

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

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