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spoIID [2019-07-10 16:58:34]
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spoIID [2019-07-10 16:58:34]

cell wall hydrolase (lytic transglycosylase), required for complete dissolution of the asymmetric septum
locus
BSU36750
pI
9.76
mw
37.25 kDa
protein length
343 aa Sequence Blast
gene length
1032 bp Sequence Blast
function
dissolution of the septal cell wall
product
lytic transglycosylase
essential
no
synonyms
spoIIC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,776,722 3,777,753

    The protein

    Catalyzed reaction/ biological activity

  • degrades the glycan strands of the peptidoglycan into disaccharide units [Pubmed|20159959], enhances [protein|C9817A54C8E72193280E393D7BD3375BD1751738|SpoIIP] activity [Pubmed|20159959]
  • required for proper localization of [protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] (together with [protein|C9817A54C8E72193280E393D7BD3375BD1751738|SpoIIP]) [Pubmed|23834622]

    • Structure

  • [PDB|4RWR] (from ''B. anthracis'', 54% identity, 74% similarity) [pubmed|27226615]

    • [SW|Localization]

  • cell membrane [Pubmed|20159959]
  • the complex of [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|SpoIID], [protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|SpoIIM], and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|SpoIIP] localizes at the leading edge of the mother cell engulfing membrane and is essential and rate limiting for membrane migration [Pubmed|20382772,12502745]

    • Expression and Regulation

    Operons

    genes
    [gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]
    description
    [Pubmed|3011962]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

    • regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|15699190,15383836]
    • view in new tab

    Biological materials

    Mutant

  • BKE36750 ([gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCTGCCTCCTGC, downstream forward: _UP4_TAGAAAAAGACGCTCATTGG
  • BKK36750 ([gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCTGCCTCCTGC, downstream forward: _UP4_TAGAAAAAGACGCTCATTGG

    • References

    Reviews

  • 23944268

    • Original publications

  • 17376078,1946462,11886548,15752199,3011962,15699190,15383836,20159959,20382772,12502745,20444098,23834622,23859254,27226615