spoIID

cell wall hydrolase (lytic transglycosylase), required for complete dissolution of the asymmetric septum

locus: BSU_36750

pI: 9.76

mw: 37.25 kDa

protein length: 343 aa

gene length: 1032 bp

function: dissolution of the septal cell wall

product: lytic transglycosylase

essential: no

synonyms: spoIIC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,776,722 3,777,753

The protein

Catalyzed reaction/ biological activity

• degrades the glycan strands of the peptidoglycan into disaccharide units [Pubmed|20159959], enhances [protein|C9817A54C8E72193280E393D7BD3375BD1751738|SpoIIP] activity [Pubmed|20159959]
• required for proper localization of [protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] (together with [protein|C9817A54C8E72193280E393D7BD3375BD1751738|SpoIIP]) [Pubmed|23834622]

Structure

• [PDB|4RWR] (from ''B. anthracis'', 54% identity, 74% similarity) [pubmed|27226615]

[SW|Localization]

• cell membrane [Pubmed|20159959]
• the complex of [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|SpoIID], [protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|SpoIIM], and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|SpoIIP] localizes at the leading edge of the mother cell engulfing membrane and is essential and rate limiting for membrane migration [Pubmed|20382772,12502745]

Expression and Regulation

Operons

• genes: [gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]
  description: [Pubmed|3011962]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|15699190,15383836]

Biological materials

Mutant

• BKE36750 ([gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCTGCCTCCTGC, downstream forward: _UP4_TAGAAAAAGACGCTCATTGG
• BKK36750 ([gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCTGCCTCCTGC, downstream forward: _UP4_TAGAAAAAGACGCTCATTGG

References

Reviews

• 23944268,32660383

Original publications

• 17376078,1946462,11886548,15752199,3011962,15699190,15383836,20159959,20382772,12502745,20444098,23834622,23859254,27226615,31282858