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sacB [2017-8-11 16:0:3]
function
utilization of sucrose, production of levan
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]Gene
Coordinates
3,536,012 → 3,537,433
The protein
Catalyzed reaction/ biological activity
Sucrose (6)-beta-D-fructofuranosyl-(2→)(n) alpha-D-glucopyranoside = glucose (6)-beta-D-fructofuranosyl-(2→)(n 1) alpha-D-glucopyranoside (according to Swiss-Prot)Protein family
glycosyl hydrolase 68 family (according to Swiss-Prot)Structure
[PDB|1PT2] (complex with sucrose), [PDB|1OYG][SW|Localization]
secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]Expression and Regulation
Operons
genes
[gene|AAA944BE7F01CE90F7730EECA16F3E4ED77D165A|sacB]-[gene|33BEC05F2129EBAF6699E847B0909A5A48F0E869|levB]
description
[Pubmed|11739774]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2428811], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|2428811], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon][protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY]: antitermination, /antitermination via binding to a [SW|RNA switch], in [regulon|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY regulon]regulation
the [SW|RNA switch] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]view in new tabBiological materials
lacZ fusion
pGP564 (in [protein|search|pAC7]), a series of RAT mutations in [protein|search|pAC7], all available in [SW|Stülke] labReferences
Reviews
Original publications
6402497,2428811,18197985,18957862,2105292,11739774,3039303,22247149,26256357,26600431,26646447,28871528