alsS [2018-07-26 12:19:50]

acetolactate synthase

Locus: BSU36010

Isoelectric point: 5.16

Molecular weight: 61.95 kDa

Protein length: 571 aa

Gene length: 1713 bp

Function: overflow metabolism

Product: acetolactate synthase

Essential: no

E.C.: 2.2.1.6

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Overflow metabolism

Gene

Coordinates: 3,709,628 → 3,711,340

The protein

Catalyzed reaction/ biological activity

• 2 pyruvate = 2-acetolactate CO₂ (according to Swiss-Prot)

Protein family

• TPP enzyme family (according to Swiss-Prot)

Paralogous protein(s)

• IlvB, YdaP

Cofactors

• Mg² PubMed
• TPP PubMed

Structure

• 4RJJ (complex wit TPP) PubMed

Expression and Regulation

Operons

• Genes: alsS-alsD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AlsR: activation, in the presence of acetate PubMed, in AlsR regulon
  • Rex: repression, if the ratio NADH2/NAD is high PubMed, in Rex regulon
  • stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript PubMed, in stringent response

  Regulation

  • induction by acetate (AlsR) PubMed
  • expression is heterogeneous PubMed
  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed

Biological materials

Mutant

• 1A979 ( alsS::cat), PubMed, available at BGSC
• BKE36010 (ΔalsS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACACCCTCACTCCTTATT, downstream forward: _UP4_TAGCACTCTGCGCATCACGA
• BKK36010 (ΔalsS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACACCCTCACTCCTTATT, downstream forward: _UP4_TAGCACTCTGCGCATCACGA

References

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