iolX

scyllo-inositol 2-dehydrogenase

locus: BSU_10850

pI: 5.30

mw: 37.33 kDa

protein length: 342 aa

gene length: 1029 bp

function: utilization of scyllo-inositol

product: scyllo-inositol 2-dehydrogenase

essential: no

synonyms: yuxD,yucG,yisS

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.16|Utilization of inositol]

Gene

Coordinates: 1,164,370 1,165,398

Phenotypes of a mutant

• impaired growth with scyllo-inositol as the only carbon source [Pubmed|20133360]

The protein

Catalyzed reaction/ biological activity

• NAD+ + scyllo-inositol --> H⁺ + NADH + scyllo-inosose (according to UniProt)

Protein family

• [SW|Gfo/Idh/MocA family] (according to UniProt)

Paralogous protein(s)

• [protein|03BE6DA854612B3F6741E0E167AB310F0DE96285|YfiI], [protein|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|NtdC], [protein|484D17480DFAD3667E0EBC6D38854A7546A8DB46|YteT], [protein|920C5750CB95102B073C32874D512E8D636D2C7D|IolU], [protein|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|IolG], [protein|942BAE9FA023DFCA06B6D26A856A72F2979E23FA|YrbE], [protein|7DFE74C751A67CCC84579D52005E1399B0A10166|IolW]

[SW|Cofactors]

• NAD⁺ [pubmed|28693424]

Structure

• [PDB|3EZY] (from Thermotoga maritima, 37% identity)

Expression and Regulation

Operons

• genes: [gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|17F5188958A415D8D09439E02E1D1E0661B5C760|IolQ]: repression, [pubmed|28693424], in [regulon|17F5188958A415D8D09439E02E1D1E0661B5C760|IolQ regulon]
  regulation: induced by scyllo-inositol [pubmed|28693424]

Biological materials

Mutant

• MGNA-B191 (yisS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1190 NBRP B. subtilis, Japan]
• BKE10850 ([gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCCCATCCTCTCCTTT, downstream forward: _UP4_TAAAAGCTAAGTATGTTGCA
• BKK10850 ([gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCCCATCCTCTCCTTT, downstream forward: _UP4_TAAAAGCTAAGTATGTTGCA

References

• 20133360,28693424