gerM

lipoprotein, germination (cortex hydrolysis) and sporulation, required for the assembly of the basal platform of the SpoIIIA-SpoIIQ trans-envelope complex during sporulation

Locus: BSU_28380

Isoelectric point: 4.68

Molecular weight: 39.41 kDa

Protein length: 366 aa

Gene length: 1101 bp

Function: germination (cortex hydrolysis) and sporulation

Product: SpoIIQ recruitment factor

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Germination → Additional germination proteins

Gene

Coordinates: 2,902,002 2,903,102

Phenotypes of a mutant

• inactivation of gerM reduces sporulation efficiency to 0.3% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• required for the assembly of the basal platform of the SpoIIIAH-SpoIIQ transenvelope complex during sporulation PubMed
• required for the recruitment of SpoIIQ to the septal membranes on the forespore side PubMed

Structure

• 6GZ8 (first GerMN domain) PubMed
• 6GZB (tandem GerMN domains) PubMed

Localization

• lipoprotein, outer forespore membrane, localization depends on SpoIIIAH and SpoIIQ PubMed

Expression and Regulation

Operons

• Genes: gerM
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

Biological materials

Mutant

• BKE28380 (gerM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTTTTCCCTCCTAAGG, downstream forward: _UP4_TAAATTGGGATTTTTGATAT
• BKK28380 (gerM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTTTTCCCTCCTAAGG, downstream forward: _UP4_TAAATTGGGATTTTTGATAT

References

Reviews

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Original Publications

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