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gyrB [2018-02-06 13:14:42]
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gyrB [2018-02-06 13:14:42]

DNA gyrase (subunit B)
locus
BSU00060
pI
5.38
mw
71.32 kDa
protein length
638 aa Sequence Blast
gene length
1914 bp Sequence Blast
function
DNA supercoiling, initation of replication cycle and DNA elongation
product
DNA gyrase (subunit B)
essential
yes
ec
5.99.1.3
synonyms
novA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    4,867 → 6,783

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to Swiss-Prot)

    • Protein family

  • type II topoisomerase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|ParE]

    • [SW|Domains]

  • [SW|TOPRIM domain] (aa 422-536) [Pubmed|9722641]

    • Modification

  • phosphorylated on Ser-400 [Pubmed|20509597]

    • Effectors of protein activity

  • novobiocin binds to the ATP binding site of GyrB and inhibits ATP hydrolysis and enzymatic activity [pubmed|6282812,368801]
  • an R138L mutation confers novobiocin resistance [pubmed|29396913]

    • Structure

  • [PDB|4I3H] (from ''Streptococcus pneumoniae'', 55% identity) [Pubmed|23965305]

    • [SW|Localization]

  • nucleoid-associated in actively growing cells [Pubmed|9539793]

    • additional information

  • GyrB is subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

    • Expression and Regulation

    Operons

    genes
    [gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
    • view in new tab

    Labs working on this gene/protein

  • [SW|Dagmar Klostermeier], University of Münster, Germany [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]

    • References

  • 17981983,9539793,2987848,19666507,17320901,20509597,23475963,23965305,9722641,27557712,27793284,12767818,368801,6282812,29396913