gyrB

DNA gyrase (subunit B)

locus: BSU_00060

pI: 5.38

mw: 71.32 kDa

protein length: 638 aa

gene length: 1917 bp

function: DNA supercoiling, control of [category|SW 3.1.1|replication] initiation

product: DNA gyrase (subunit B)

essential: yes

ec: 5.99.1.3

synonyms: novA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 4,867 6,783

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)
• decreases [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] association with the oriC and inhibits [category|SW 3.1.1|replication] initiation [pubmed|29396913]

Protein family

• type II topoisomerase [protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|GyrB] family (together with [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|ParE]) (according to UniProt)

Paralogous protein(s)

• [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|ParE]

[SW|Domains]

• [SW|TOPRIM domain] (aa 422-536) [Pubmed|9722641]

Modification

• phosphorylated on Ser-400 [Pubmed|20509597]

Effectors of protein activity

• novobiocin binds to the ATP binding site of GyrB and inhibits ATP hydrolysis and enzymatic activity, this results in [category|SW 3.1.1|replication] overinitiation [pubmed|29396913,6282812,368801]
• an R138L mutation confers novobiocin resistance [pubmed|29396913]

Structure

• [PDB|4I3H] (from ''Streptococcus pneumoniae'', 55% identity) [Pubmed|23965305]

[SW|Localization]

• nucleoid-associated in actively growing cells [Pubmed|9539793]

additional information

• GyrB is subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operons

• genes: [gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

labs

• [SW|Dagmar Klostermeier], University of Mnster, Germany [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]

References

Reviews

• 16936698,29772727

Original publications

• 17981983,9539793,2987848,19666507,17320901,20509597,23475963,23965305,9722641,27557712,27793284,12767818,368801,6282812,29396913,8940171,7590259,10944214,6248518,6243420,225110,17334374,30517674,31953321