gltB [2018-02-13T09:44:33.000Z]

small subunit of glutamate synthase

locus: BSU18440

pI: 7.70

mw: 54.78 kDa

protein length: 493 aa

gene length: 1479 bp

function: glutamate biosynthesis

product: glutamate synthase (small subunit)

essential: no

ec: 1.4.1.13

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]

Gene

Coordinates: 2,008,572 → 2,010,053

Phenotypes of a mutant

• auxotrophic for glutamate

The protein

Catalyzed reaction/ biological activity

• 2 L-glutamate NADP = L-glutamine 2-oxoglutarate NADPH (according to Swiss-Prot) 2 L-glutamate NADP( ) <=> L-glutamine 2-oxoglutarate NADPH

Protein family

• glutamate synthase family.

Paralogous protein(s)

• none

[SW|Domains]

• nucleotide binding domain (NADP) (299–313)

Structure

• [PDB|2VDC] (the [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|GltA]-[protein|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|GltB] complex of ''Azospirillum brasiliense'') [Pubmed|18199747]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]
  description: [Pubmed|12823818]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2548995], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|11029411], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|87BCAE725B02860156D50E1783F6DB68510C811E|GltC]: activation, [Pubmed|2548995], in [regulon|87BCAE725B02860156D50E1783F6DB68510C811E|GltC regulon]
  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: indirect effect, in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • expressed in the presence of ammonium [Pubmed|11029411]

Other regulations

• [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]: translation inhibition,

Biological materials

Mutant

• GP807 (del ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]''::''tet'') , available in [SW|Jörg Stülke]'s lab
• GP517 (ermC), available in [SW|Jörg Stülke]'s lab
• BKE18440 (Δ[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT, downstream forward: _UP4_TAAATAAAGGGGATTATCAT
• BKK18440 (Δ[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT, downstream forward: _UP4_TAAATAAAGGGGATTATCAT

Expression vector

• pGP1119 (in [SW|pGP380], for SPINE, expression in ''B. subtilis''), available in [SW|Jörg Stülke]'s lab [pubmed|20933603]

lacZ fusion

• see ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
• [SW|Fabian Commichau] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

References

Reviews

• 16143852,12859215,22625175,12859215

Original publications

• 27223617,12823818,20933603,11029411,7559360,15150225,2548995,17183217,17608797,17134717,14523131,12823818,18326565,18199747,22389480,25755103,28294562,29242163