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gltB [Sat May 28 2016 00:19:29 GMT+0200 (CEST)]
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gltB [Sat May 28 2016 00:19:29 GMT+0200 (CEST)]

small subunit of glutamate synthase
Locus
BSU18440
Isoelectric point
7.00
Molecular weight
54.00 kDa
Function
glutamate biosynthesis
Product
glutamate synthase (small subunit)
Essential
no
E.C.
1.4.1.13
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation

    • Categories containing this gene/protein

  • biosynthesis/ acquisition of amino acids, glutamate metabolism

    • This gene is a member of the following regulons

  • GltC regulon, FsrA regulon, TnrA regulon

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,008,572 -> 2,010,053

    Phenotypes of a mutant

  • auxotrophic for glutamate

    • The protein

    Catalyzed reaction/ biological activity

  • 2 L-glutamate NADP = L-glutamine 2-oxoglutarate NADPH (according to Swiss-Prot) 2 L-glutamate NADP( ) <=> L-glutamine 2-oxoglutarate NADPH

    • Protein family

  • glutamate synthase family.

    • Paralogous protein(s)

  • none

    • Domains

  • nucleotide binding domain (NADP) (299–313)

    • Structure

  • 2VDC (the GltA-GltB complex of Azospirillum brasiliense) PubMed

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Interactions

  • GltA-GltB PubMed

    • Expression and Regulation

    Operon

  • gltA-gltB PubMed

    • Sigma factor

  • SigA PubMed

    • Regulation

  • see ''gltA

    • Biological materials

    Mutant

  • GP807 (del gltA-gltB::tet) , available in Jörg Stülke's lab
  • GP517 (ermC), available in Jörg Stülke's lab

    • Expression vector

  • pGP1119 (in pGP380, for SPINE, expression in B. subtilis), available in Jörg Stülke's lab

    • LacZ fusion

  • see ''gltA

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
  • Jörg Stülke, University of Göttingen, Germany
  • Homepage
  • Fabian Commichau University of Göttingen, Germany
  • Homepage

    • References

    Reviews

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    Original publications

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