psdS

two-component sensor kinase, control of [gene|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA]-[gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB] in response to lipid II-binding lantibiotics, such as nisin and gallidermin

locus: BSU_34710

pI: 7.36

mw: 40.02 kDa

protein length: 356 aa

gene length: 1071 bp

function: resistance against toxic peptides

product: two-component sensor kinase

essential: no

synonyms: yvcQ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 3,566,358 3,567,428

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|985E7F25C809AA5DF372101E64960DF704EED53F|PsdR]
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

[SW|Domains]

• two transmembrane segments
• [SW|Histidine kinase domain] (aa 133-349) (according to UniProt)

Modification

• autophosphorylation on a His residue

Structure

• [PDB|4I5S] (from Streptococcus mutans, the C-terminal histidine phosphotransferase domain, 29% identity) [pubmed|23468592]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|985E7F25C809AA5DF372101E64960DF704EED53F|psdR]-[gene|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|psdS]-[gene|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA]-[gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB]
  description: [Pubmed|21078927]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21078927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|985E7F25C809AA5DF372101E64960DF704EED53F|PsdR]: activation, [Pubmed|21078927], in [regulon|985E7F25C809AA5DF372101E64960DF704EED53F|PsdR regulon]

  regulation

  • ''[protein|search|psdA]'': induced by nisin, gallidermin ([protein|search|PsdR]) [Pubmed|21078927]

Biological materials

Mutant

• MGNA-B636 (yvcQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1635 NBRP B. subtilis, Japan]
• BKE34710 ([gene|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|psdS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAGCGGTCGATGAGATAAG, downstream forward: _UP4_TAAGATTGGGGAGCGGAATT
• BKK34710 ([gene|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|psdS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAGCGGTCGATGAGATAAG, downstream forward: _UP4_TAAGATTGGGGAGCGGAATT

References

• 10094672,18394148,14651641,21283517,27188294,21078927,23468592