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yugT [2018-09-26 10:49:50]
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yugT [2018-09-26 10:49:50]

similar to exo-alpha-1,4-glucosidase
Locus
BSU31290
Isoelectric point
4.97
Molecular weight
63.82 kDa
Protein length
554 aa Sequence Blast
Gene length
1662 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
E.C.
3.2.1.10
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,214,372 → 3,216,036

The protein

Catalyzed reaction/ biological activity

  • Hydrolysis of (1→6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose (according to Swiss-Prot)
  • Protein family

  • glycosyl hydrolase 13 family (according to Swiss-Prot)
  • Structure

  • 2ZE0 (from Geobacillus sp., 64% identity), PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • Spx: activation, PubMed, in Spx regulon
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    Biological materials

    Mutant

  • MGNA-B547 (yugT::erm), available at the NBRP B. subtilis, Japan
  • BKE31290 (ΔyugT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAGCCACTCCTTCCCA, downstream forward: _UP4_TAAACTCCAGCCTTAGACTG
  • BKK31290 (ΔyugT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAGCCACTCCTTCCCA, downstream forward: _UP4_TAAACTCCAGCCTTAGACTG
  • References

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