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yrpB [2019-08-08 08:31:05]
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yrpB [2019-08-08 08:31:05]

similar to 2-nitropropane dioxygenase
locus
BSU_26800
pI
5.26
mw
37.91 kDa
protein length
347 aa Sequence Blast
gene length
1044 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    2,736,915 2,737,958

    The protein

    Catalyzed reaction/ biological activity

  • 2 2-nitropropane + O2 = 2 acetone + 2 nitrite (according to Swiss-Prot)
  • Protein family

  • nitronate monooxygenase family (single member, according to UniProt)
  • Structure

  • [PDB|4QIS] (from Pseudomonas Aeruginosa 36% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|A936E6339085F1B9494405243DD801DDB4D8A2CC|yrpB]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A866 (yrpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/866 NBRP B. subtilis, Japan]
  • BKE26800 ([gene|A936E6339085F1B9494405243DD801DDB4D8A2CC|yrpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCGAATCCTCCCGTTA, downstream forward: _UP4_TAGGCGCACCTCATTGATAG
  • BKK26800 ([gene|A936E6339085F1B9494405243DD801DDB4D8A2CC|yrpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCGAATCCTCCCGTTA, downstream forward: _UP4_TAGGCGCACCTCATTGATAG