SubtiBank SubtiBank
yrpB [2019-08-08 08:31:05]
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yrpB [2019-08-08 08:31:05]

similar to 2-nitropropane dioxygenase
locus
BSU_26800
pI
5.26
mw
37.91 kDa
protein length
347 aa Sequence Blast
gene length
1044 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    2,736,915 2,737,958

    The protein

    Catalyzed reaction/ biological activity

  • 2 2-nitropropane + O2 = 2 acetone + 2 nitrite (according to Swiss-Prot)

    • Protein family

  • nitronate monooxygenase family (single member, according to UniProt)

    • Structure

  • [PDB|4QIS] (from Pseudomonas Aeruginosa 36% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|A936E6339085F1B9494405243DD801DDB4D8A2CC|yrpB]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A866 (yrpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/866 NBRP B. subtilis, Japan]
  • BKE26800 ([gene|A936E6339085F1B9494405243DD801DDB4D8A2CC|yrpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCGAATCCTCCCGTTA, downstream forward: _UP4_TAGGCGCACCTCATTGATAG
  • BKK26800 ([gene|A936E6339085F1B9494405243DD801DDB4D8A2CC|yrpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCGAATCCTCCCGTTA, downstream forward: _UP4_TAGGCGCACCTCATTGATAG