upp

uracil phosphoribosyltransferase

locus: BSU_36890

pI: 5.86

mw: 22.89 kDa

protein length: 209 aa

gene length: 630 bp

function: UMP synthesis

product: uracil phosphoribosyltransferase

essential: no

ec: 2.4.2.9

synonyms: ipc-35d

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]

Gene

Coordinates: 3,788,426 3,789,055

The protein

Catalyzed reaction/ biological activity

• diphosphate + UMP --> 5-phospho-α-D-ribose 1-diphosphate + uracil (according to UniProt)

Protein family

• UPRTase family (single member, according to UniProt)

Structure

• [PDB|2EHJ] (from ''Escherichia coli'', 52% identity, 73% similarity)

Expression and Regulation

Operons

• genes: [gene|A92A17CAE2EF33C320B958325F9728EA0FD98F85|upp]
  description: [Pubmed|9353933]

Biological materials

Mutant

• 1A832 (no resistance), [Pubmed|12366828], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A832&Search=1A832 BGSC]
• BKE36890 ([gene|A92A17CAE2EF33C320B958325F9728EA0FD98F85|upp]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGTGTTTCAGCTCCT, downstream forward: _UP4_TAAAAAATGAAATCCCCAAA
• BKK36890 ([gene|A92A17CAE2EF33C320B958325F9728EA0FD98F85|upp]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGTGTTTCAGCTCCT, downstream forward: _UP4_TAAAAAATGAAATCCCCAAA

References

• 7798145