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tcyK [2018-05-04 19:27:35]
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tcyK [2018-05-04 19:27:35]

cystine ABC transporter (binding protein)
Locus
BSU29370
Isoelectric point
9.01
Molecular weight
30.09 kDa
Protein length
270 aa Sequence Blast
Gene length
810 bp Sequence Blast
Function
cystine uptake
Product
cystine ABC transporter (binding protein)
Essential
no
Synonyms
ytmK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → ABC transporters → Importers → Uptake of amino acids
  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,006,600 → 3,007,412

    The protein

    Protein family

  • bacterial solute-binding protein 3 family (according to Swiss-Prot)

    • Structure

  • 2O1M

    • Localization

  • attached to the membrane via TcyL-TcyM PubMed

    • Expression and Regulation

    Operons

    Genes
    snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • AscR: activation, PubMed, in AscR regulon

    • Regulation

  • induced in the presence of methionine and taurine PubMed
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    Biological materials

    Mutant

  • MGNA-A171 (ytmK::erm), available at the NBRP B. subtilis, Japan
  • 1A948 ( tcyK::kan), PubMed, available at BGSC
  • BKE29370 (ΔtcyK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCGTTTTCATCTCATCGC, downstream forward: _UP4_TAGGTAAAAAGAGAGGGAGC
  • BKK29370 (ΔtcyK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCGTTTTCATCTCATCGC, downstream forward: _UP4_TAGGTAAAAAGAGAGGGAGC

    • References

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