tcyK

cystine ABC transporter (binding protein)

Locus: BSU_29370

Isoelectric point: 9.01

Molecular weight: 30.09 kDa

Protein length: 270 aa

Gene length: 813 bp

Function: cystine uptake

Product: cystine ABC transporter (binding protein)

Essential: no

Synonyms: ytmK

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of amino acids
• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of cysteine
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,006,600 3,007,412

The protein

Protein family

• bacterial solute-binding protein 3 family (according to UniProt)

Paralogous protein(s)

• TcyJ

Structure

• 2O1M

Localization

• attached to the membrane via TcyL-TcyM PubMed

Expression and Regulation

Operons

• Genes: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • AscR: activation, PubMed, in AscR regulon

  Regulation

  • induced in the presence of methionine and taurine PubMed

Biological materials

Mutant

• MGNA-A171 (ytmK::erm), available at the NBRP B. subtilis, Japan
• 1A948 ( tcyK::kan), PubMed, available at BGSC
• BKE29370 (tcyK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCGTTTTCATCTCATCGC, downstream forward: _UP4_TAGGTAAAAAGAGAGGGAGC
• BKK29370 (tcyK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCGTTTTCATCTCATCGC, downstream forward: _UP4_TAGGTAAAAAGAGAGGGAGC

References

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