SubtiBank SubtiBank
ydiS
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ydiS

component of the BsuM DNA restriction system, similar to 5-methylcytosine-specific restriction enzyme
Locus
BSU_06100
Isoelectric point
8.70
Molecular weight
39.88 kDa
Protein length
343 aa Sequence Blast
Gene length
1032 bp Sequence Blast
Function
BsuM DNA restriction
Product
unknown
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
660,596 661,627

The protein

Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates (according to UniProt)
  • Structure

  • 6HZ4 (5-methylcytosine-specific restriction enzyme from E. coli, corresponds to aa 29 ... 223, 29% identity) PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-C208 (ydiS::erm), available at the NBRP B. subtilis, Japan
  • DELTA0 (ydiS trpC2), available at BGSC
  • BKE06100 (ydiS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT, downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
  • BKK06100 (ydiS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT, downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
  • References

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