ydiS

component of the BsuM DNA restriction system, similar to 5-methylcytosine-specific restriction enzyme

locus: BSU_06100

pI: 8.70

mw: 39.88 kDa

protein length: 343 aa

gene length: 1032 bp

function: BsuM DNA restriction

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.4|DNA restriction/ modification]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.5|Prophage 3]

Gene

Coordinates: 660,596 661,627

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates (according to UniProt)

Structure

• [PDB|6HZ4] (5-methylcytosine-specific restriction enzyme from E. coli, corresponds to aa 29 ... 223, 29% identity) [pubmed|31296862]

Expression and Regulation

Operons

• genes: [gene|4BD4CFB10700907DF1BBF3C9CFACAF573588E85F|ydiR]-[gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]-[gene|D36B91B3C8EAFEB80A7395C62A50A5F80B8CC5C5|ydjA]
  description: [Pubmed|11751814]

Biological materials

Mutant

• MGNA-C208 (ydiS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2206 NBRP B. subtilis, Japan]
• DELTA0 (''ydiS trpC2''), available at BGSC
• BKE06100 ([gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT, downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
• BKK06100 ([gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT, downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA

References

• 11751814,22092861,31296862