xlyB

N-acetylmuramoyl-L-alanine amidase

Locus: BSU_12460

Isoelectric point: 9.80

Molecular weight: 33.71 kDa

Protein length: 317 aa

Gene length: 954 bp

Function: PBSX prophage-mediated lysis

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms: yjpB

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
• Prophages and mobile genetic elements → Prophages → PBSX prophage
• Prophages and mobile genetic elements → Prophages → Phage-related functions
• Groups of genes → Secreted proteins

Gene

Coordinates: 1,317,535 1,318,488

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)

Paralogous protein(s)

• CwlA, CwlH, XlyA

Domains

• contains a N-acetylglucosamine-polymer-binding LysM domain PubMed
• contains an amidase_2 domain (like BlyA, CwlA, CwlH, XlyB)
• N-acetylmuramoyl-L-alanine amidase domain (aa 40-142) (according to UniProt)
• LysM domain (aa 177-221) (according to UniProt)

Structure

• 2L47 (from Bacillus phage Gamma, corresponds to aa 3 ... 166, 57% identity)

Localization

• secreted (according to UniProt)

Expression and Regulation

Operons

• Genes: xlyB
  Description: PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• BKE12460 (xlyB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT
• BKK12460 (xlyB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT

References

Reviews

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Original publications

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