adeC [2019-06-24 09:14:11]

adenine deaminase

locus: BSU14520

pI: 4.72

mw: 62.64 kDa

protein length: 577 aa

gene length: 1734 bp

function: utilization of adenine as nitrogen source,purine salvage and interconversion

product: adenine deaminase

essential: no

ec: 3.5.4.2

synonyms: ade,yzaD

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

Gene

Coordinates: 1,521,351 1,523,084

The protein

Catalyzed reaction/ biological activity

• Adenine + H₂O = hypoxanthine + NH₃ (according to Swiss-Prot)

Protein family

• [SW|Metallo-dependent hydrolases superfamily] (according to UniProt)
• Adenine deaminase family (with [protein|A85EB6E97AC9B2CA46BA6EF315BDA72A035DD3E7|AdeC], according to UniProt)

Structure

• [PDB|3NQB] (from Agrobacterium tumefaciens, 31% identity)

Expression and Regulation

Operons

• genes: [gene|A85EB6E97AC9B2CA46BA6EF315BDA72A035DD3E7|adeC]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE14520 ([gene|A85EB6E97AC9B2CA46BA6EF315BDA72A035DD3E7|adeC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATAGGCCCCTCCTTTTT, downstream forward: _UP4_TAAAAAGAGGCACTCCCTTA
• BKK14520 ([gene|A85EB6E97AC9B2CA46BA6EF315BDA72A035DD3E7|adeC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATAGGCCCCTCCTTTTT, downstream forward: _UP4_TAAAAAGAGGCACTCCCTTA

References

• 8550522