scoA

succinyl CoA:3-oxoacid CoA-transferase (subunit A)

locus: BSU_38990

pI: 6.61

mw: 25.25 kDa

protein length: 238 aa

gene length: 717 bp

function: 3-hydroxybutyrate utilization

product: succinyl CoA:3-oxoacid CoA-transferase (subunit A)

essential: no

ec: 2.8.3.5

synonyms: yxjD

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 4,001,976 4,002,692

The protein

Catalyzed reaction/ biological activity

• 3-oxo acid + succinyl-CoA --> 3-oxoacyl-CoA + succinate (according to UniProt)

Protein family

• 3-oxoacid CoA-transferase subunit A family (with [protein|D8A050BF150C6E5B910C24C0E023F0829EC95BAD|YodS], according to UniProt)

Paralogous protein(s)

• [protein|D8A050BF150C6E5B910C24C0E023F0829EC95BAD|YodS]

Structure

• [PDB|3CDK] (the [protein|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|ScoA]-[protein|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|ScoB] complex)

Expression and Regulation

Operons

• genes: [gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]-[gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]-[gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]-[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135,10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|12662922]

Biological materials

Mutant

• MGNA-B728 (yxjD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1727 NBRP B. subtilis, Japan]
• BKE38990 ([gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATGCCCCTTTCGT, downstream forward: _UP4_GCATCGGGAAAGGGTGAGGC
• BKK38990 ([gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATGCCCCTTTCGT, downstream forward: _UP4_GCATCGGGAAAGGGTGAGGC

References

• 17363272,10746760,16672620,12662922,12850135,10666464