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pfkA [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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pfkA [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

phosphofructokinase, glycolytic enzyme
locus
BSU29190
pI
6.00
mw
34.00 kDa
function
catabolic enzyme in glycolysis
product
6-phosphofructokinase
essential
no
ec
2.7.1.11
synonyms
pfk
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|phosphoproteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,986,588 -> 2,987,547

    Phenotypes of a mutant

  • essential according to [Pubmed|12682299], non-essential according to [Pubmed|23420519,20473954]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate (according to Swiss-Prot)

    • Protein family

  • phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family

    • Kinetic information

  • Allosteric Regulation (Reversible) [Pubmed|4269800]

    • [SW|Domains]

  • 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)

    • Modification

  • phosphorylated on Arg-233 [Pubmed|22517742]

    • [SW|Cofactors]

  • Mg2

    • Effectors of protein activity

  • Inhibited by citrate, PEP (Hill Coefficient 3) and Ca2 (competes with Mg2) in ''B. licheniformes'' [Pubmed|4269800].
  • Inhibited by ATP (competitively) and f6p (non-competitively) in ''G. stearothermophillus'' [Pubmed|8136379]
  • Activated by GDP and ADP in lower concentrations (1mM); above that inhibition, competing with the ATP for the binding site (in ''G. stearothermophillus'') [Pubmed|7873536]
  • Activated by NH4 [Pubmed|7873536]

    • Structure

  • [PDB|4A3S] [Pubmed|22198292]
  • [PDB|1MTO] (mutant, complex with fructose-6-phosphate, ''Geobacillus stearothermophilus'')
  • [PDB|4PFK] (''Geobacillus stearothermophilus'')

    • [SW|Localization]

  • cytoplasm (Homogeneous) [Pubmed|16479537]

    • [SW|Interactions]

  • forms homotetramers [Pubmed|22198292]
  • part of the [SW|RNA degradosome] [Pubmed|19193632]
  • [protein|search|PfkA]-[protein|search|Pgm] [Pubmed|19193632]
  • [protein|search|PfkA]-[protein|search|Eno] [Pubmed|19193632], K(D) of the interaction: 40 nM [Pubmed|22198292]
  • [protein|search|PfkA]-[protein|search|Rny] [Pubmed|19193632,21803996]
  • [protein|search|PfkA]-[protein|search|PnpA] [Pubmed|19193632]
  • [protein|search|PfkA]-[protein|search|RnjA] [Pubmed|19193632],

    • Additional information

  • PfkA is a [SW|moonlighting protein]. [Pubmed|19193632]
  • extensive information on the structure and enzymatic properties of PfkA can be found at [http://www.proteopedia.org/wiki/index.php/Phosphofructokinase_(PFK) Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|pfkA]-[protein|search|pyk]-[protein|search|ytzA]'' [Pubmed|11489127]

    • Regulation

  • twofold induced by glucose [Pubmed|11489127]

    • Biological materials

    Mutant

  • GP590 (''pfkA''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP595 (''pfkA''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP1744: ''pfkA::aphA3'', available in [SW|Jörg Stülke]' lab

    • Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP87, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], in [SW|pGP382]: pGP1266, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP393, available in [SW|Jörg Stülke]'s lab
  • for expression in ''B. subtilis'', in [SW|pBQ200]: pGP1422, available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP511 (in [protein|search|pAC6]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1019 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

    • References

  • 16479537,19193632,11489127,8136379,7873536,4269800,20473954,20572937,21803996,22198292,22198292,22517742,23420519