padR

repressor of yveF-yveG-padC, induction occurs by binding of phenolic acids,stress response

Locus: BSU_08340

Isoelectric point: 7.23

Molecular weight: 21.08 kDa

Protein length: 182 aa

Gene length: 549 bp

Function: regulation of the phenolic acid stress response

Product: transcriptional repressor

Essential: no

Synonyms: yfiO

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Lifestyles → Coping with stress → Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

Gene

Coordinates: 909,198 909,746

The protein

Domains

• N-terminal winged helix-turn-helix (wHTH) domain (NTD) PubMed
• C-terminal homodimerization domain (CTD) PubMed

Effectors of protein activity

• phenolic acid acts as molecular inducer and releases PadR from the yveF-yveG-padC promoter PubMed

Structure

• 5X12 (PadR) PubMed
• 5X11 (complex with dsDNA) PubMed
• 5X13 (complex with p-coumaric acid) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: padR
  Description: PubMed

  Regulation

  • weak constitutive expression

Biological materials

Mutant

• MGNA-C303 (yfiO::erm), available at the NBRP B. subtilis, Japan
• BKE08340 (padR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTATCCTCCGTGTCTG, downstream forward: _UP4_TAACCGCAGTTCAGGCTGCA
• BKK08340 (padR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTATCCTCCGTGTCTG, downstream forward: _UP4_TAACCGCAGTTCAGGCTGCA

References

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