yetM

FAD-dependent monooxygenase

locus: BSU_07230

pI: 5.34

mw: 40.82 kDa

protein length: 369 aa

gene length: 1110 bp

function: unknown

product: FAD-dependent monooxygenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 790,318 791,427

The protein

[SW|Cofactors]

• FAD (according to UniProt) [Pubmed|21635694]

Structure

• [PDB|4H2N] (from Mesorhizobium japonicum, 30% identity)

Expression and Regulation

Operons

• genes: [gene|A70EA4AEBF46ED2279E8BCE8A28439078484B6E3|yetM]
  description: [Pubmed|19329649]

  regulatory mechanism

  • [protein|BFAFC2CFA7F2FDA639B367C579E2A46242BA3DBD|YetL]: repression, [Pubmed|19329649], in [regulon|BFAFC2CFA7F2FDA639B367C579E2A46242BA3DBD|YetL regulon]

  regulation

  • induced by flavonoids of kaempferol, apigenin, and luteolin ([protein|search|YetL])[Pubmed|19329649]

Biological materials

Mutant

• MGNA-B464 (yetM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1463 NBRP B. subtilis, Japan]
• BKE07230 ([gene|A70EA4AEBF46ED2279E8BCE8A28439078484B6E3|yetM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAATAACCACCTTTT, downstream forward: _UP4_TAAATAGGAAAAGTCCAGAA
• BKK07230 ([gene|A70EA4AEBF46ED2279E8BCE8A28439078484B6E3|yetM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAATAACCACCTTTT, downstream forward: _UP4_TAAATAGGAAAAGTCCAGAA

References

• 19329649,19329649