yflA [2020-10-27 12:20:27]

general stress protein, similar to amino acid carrier protein, required for protection against paraquat stress

locus: BSU_07750

pI: 9.86

mw: 50.48 kDa

protein length: 471 aa

gene length: 1416 bp

function: protection against paraquat stress

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.3|Alanine or glycine cation symporter family]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.12|Other transporters]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 844,770 846,185

The protein

Protein family

• [SW|Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family] (according to UniProt)

Paralogous protein(s)

• [protein|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|AlsT], [protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|GlnT], [protein|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|YrbD]

Structure

• [PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 37% identity) [pubmed|30659158]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh]: repression, in [regulon|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

• genes: [gene|EA62F1DF6BB9204BB7A6B0CC6A05F63D711A451F|yflD]-[gene|530844B354D43308CC8CB7DC30DB9731A5D698EB|yflB]-[gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• GP3557 Δ[gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::phleo, available in [SW|Jörg Stülke]'s lab
• MGNA-C257 (yflA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2255 NBRP B. subtilis, Japan]
• BKE07750 ([gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07750 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATCCCATCACCCTTTTTTC, downstream forward: _UP4_TCCTAGTGTTTTGCTTTTGC
• BKK07750 ([gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCATCACCCTTTTTTC, downstream forward: _UP4_TCCTAGTGTTTTGCTTTTGC

References

• 15805528,20817675,22582280,30659158