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yflA
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yflA

general stress protein, similar to amino acid carrier protein, required for protection against paraquat stress
Locus
BSU_07750
Isoelectric point
9.86
Molecular weight
50.48 kDa
Protein length
471 aa Sequence Blast
Gene length
1416 bp Sequence Blast
Function
protection against paraquat stress
Product
unknown
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Amino acid transporters → Alanine or glycine cation symporter family
  • Cellular processes → Transporters → Transporters/ other → Other transporters
  • Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
  • Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    844,770 846,185

    The protein

    Protein family

  • Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family (according to UniProt)

    • Paralogous protein(s)

  • AlsT, GlnT, YrbD

    • Structure

  • 6CSE (sodium/alanine symporter from Methanococcus maripaludis S2, 37% identity) PubMed

    • Localization

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    yflA
    Description
    PubMed

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

    • Regulatory mechanism

  • Abh: repression, in Abh regulon
  • AbrB: repression, in AbrB regulon
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    Genes
    yflD-yflB-yflA
    Description
    PubMed

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

    • Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
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    Biological materials

    Mutant

  • GP3557 (ΔyflA::phleo), available in Jörg Stülke's lab
  • MGNA-C257 (yflA::erm), available at the NBRP B. subtilis, Japan
  • BKE07750 (yflA::erm trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CATCCCATCACCCTTTTTTC, downstream forward: _UP4_TCCTAGTGTTTTGCTTTTGC
  • BKK07750 (yflA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCATCACCCTTTTTTC, downstream forward: _UP4_TCCTAGTGTTTTGCTTTTGC

    • References

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