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yflA
general stress protein, similar to amino acid carrier protein, required for protection against paraquat stress
function
protection against paraquat stress
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.3|Alanine or glycine cation symporter family][category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.12|Other transporters][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
844,770 846,185
The protein
Protein family
[SW|Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family] (according to UniProt)Paralogous protein(s)
[protein|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|AlsT], [protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|GlnT], [protein|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|YrbD] Structure
[PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 37% identity) [pubmed|30659158][SW|Localization]
cell membrane (according to UniProt)Expression and Regulation
Operons
genes
[gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]
description
[pubmed|22383849]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]regulatory mechanism
[protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh]: repression, in [regulon|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh regulon][protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]view in new tabgenes
[gene|EA62F1DF6BB9204BB7A6B0CC6A05F63D711A451F|yflD]-[gene|530844B354D43308CC8CB7DC30DB9731A5D698EB|yflB]-[gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]
description
[pubmed|22383849]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]view in new tabBiological materials
Mutant
GP3557 (Δ[gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::phleo), available in [SW|Jörg Stülke]'s labMGNA-C257 (yflA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2255 NBRP B. subtilis, Japan]BKE07750 ([gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07750 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATCCCATCACCCTTTTTTC, downstream forward: _UP4_TCCTAGTGTTTTGCTTTTGCBKK07750 ([gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCATCACCCTTTTTTC, downstream forward: _UP4_TCCTAGTGTTTTGCTTTTGCReferences
15805528,20817675,22582280,30659158