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nifS [2019-01-04 15:10:34]
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nifS [2019-01-04 15:10:34]

cysteine desulfurase, required for NAD biosynthesis
Locus
BSU27880
Isoelectric point
6.43
Molecular weight
43.63 kDa
Protein length
395 aa Sequence Blast
Gene length
1185 bp Sequence Blast
Function
NAD biosynthesis
Product
cysteine desulfurase
Essential
no
E.C.
2.8.1.7
Synonyms
iscS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of NAD(P)

    • Gene

    Coordinates
    2,849,569 → 2,850,756

    The protein

    Catalyzed reaction/ biological activity

  • L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)

    • Protein family

  • Class-V pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • YrvO, YcbU, NifZ, SufS

    • Cofactors

  • pyridoxalphosphate PubMed

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    nifS-nadR
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • NadR: repression, PubMed, in NadR regulon

    • Regulation

  • repressed in the presence of nicotinic acid (NadR) PubMed
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    Biological materials

    Mutant

  • BKE27880 (ΔnifS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGGTCTTCCTCCATCC, downstream forward: _UP4_ATATTGACCGAAGAATTAAA
  • BKK27880 (ΔnifS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGGTCTTCCTCCATCC, downstream forward: _UP4_ATATTGACCGAAGAATTAAA

    • References

    Reviews

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    Original publications

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