ytoQ [2020-02-20 11:53:14]

unknown

locus: BSU_29850

pI: 5.77

mw: 16.65 kDa

protein length: 148 aa

gene length: 447 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 3,054,746 3,055,192

Expression and Regulation

Operons

• genes: [gene|A66C34183025AFAD7D935C7B7E107BEE94F9B362|ytoQ]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B532 (ytoQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1531 NBRP B. subtilis, Japan]
• BKE29850 ([gene|A66C34183025AFAD7D935C7B7E107BEE94F9B362|ytoQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAAAAATCCCTCCTT, downstream forward: _UP4_TAAGAAAAAAGCTTGTCGAT
• BKK29850 ([gene|A66C34183025AFAD7D935C7B7E107BEE94F9B362|ytoQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAAAAATCCCTCCTT, downstream forward: _UP4_TAAGAAAAAAGCTTGTCGAT

Expression vectors

• IPTG inducible expression of Strep-ytoQ in E. coli: pBP641 (in [SW|pGP172]) and pBP642 (in [SW|pGP574]), available in [SW|Fabian Commichau]'s lab
• Constitutive expression of ytoQ in B. subtilis: pBP639 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab, [Pubmed|29027347]
• pBP770: expression of Strep-[gene|search|ytoQ ]by pGP380 in B. subtilis suitable for SPINE, available in [SW|Jörg Stülke's] and [SW|Fabian Commichau]'s labs
• pBP779: expression of [[gene|ytoQ ]-Strep]by pGP382 in B. subtilis suitable for SPINE, available in [SW|Jörg Stülke's] and [SW|Fabian Commichau]'s labs

lacZ fusion

• pBP638 (in [SW|pAC7]), available in [SW|Fabian Commichau]'s lab [Pubmed|29027347]

labs

• [SW|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]

References

Research papers

• 11918677,29027347