two-component sensor kinase, phosphorylates Spo0F and Spo0A, part of the phosphorelay, governs expression of genes involved in biofilm formation
function
initiation of sporulation
product
two-component sensor kinase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue][SW|Categories] containing this gene/protein
[SW|protein modification], [SW|transcription factors and their control], [SW|phosphorelay], [SW|biofilm formation], [SW|membrane proteins], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|Spo0A regulon]Gene
Phenotypes of a mutant
defective in [SW|biofilm formation] [Pubmed|22882210]The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [SW|Spo0F] as part of the [SW|phosphorelay], but also direct phosphorylation of [SW|Spo0A] [Pubmed|19114652]mainly active in the younger, outer regions of a colony (with [protein|search|KinD]) [Pubmed|21097618]phosphorylates [SW|Spo0A] in response to the presence of surfactin [Pubmed|22882210], this has been refuted [Pubmed|25701730]required for initiation of [SW|sliding] together with [protein|search|KinB] [Pubmed|26152584][SW|Domains]
two transmembrane segments[SW|PAS domain]C-terminal histidine phosphotransferase domainModification
autophosphorylation on a His residueEffectors of protein activity
activity is triggered by potassium leakage [Pubmed|19114652], this has been refuted [Pubmed|25701730]activity is triggered by polyisoprenoid lipids formed by [protein|search|YisP] [Pubmed|20713508]activity is stimulated by the interaction with [protein|search|FloT] [Pubmed|26297017][SW|Localization]
cell membrane (Heterogeneous) [Pubmed|16479537]co-localizes with [protein|search|FloT] in discrete foci in the membrane [Pubmed|20713508]the localization of [protein|search|KinC] in membrane microdomains depends on [protein|search|FloA] and [protein|search|FloT] [Pubmed|22882210], this has been refuted [Pubmed|25701730][SW|Interactions]
[protein|search|KinC]-[SW|Spo0A] [Pubmed|19114652][protein|search|KinC]-[SW|Spo0F][protein|search|FloT]-[protein|search|KinC] to stimulate [protein|search|KinC] dimerization and activity [Pubmed|26297017]Expression and Regulation
Operon
kinC (according to [http://dbtbs.hgc.jp/COG/prom/kinC.html DBTBS])[SW|Sigma factor]
[protein|search|SigA] [Pubmed|8002614]Regulation
expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|8002615]Regulatory mechanism
[SW|Spo0A]: transcription activation [Pubmed|8002615]Biological materials
Mutant
1A632 ( ''kinC''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A632&Search=1A632 BGSC]BAL393 (''kinC''::''spc'')[Pubmed|26152584]References
Reviews
Original publications
26152584,19114652,10094672,11069677,16166384,20713508,8002615,16479537,8002614,20946851,20971918,21097618,22882210,23927765,25701730,26297017,26152584 
