cydC

[SW|ABC transporter] required for expression of cytochrome bd (ATP-binding protein)

locus: BSU_38740

pI: 8.51

mw: 62.63 kDa

protein length: 567 aa

gene length: 1704 bp

function: required for expression of cytochrome bd

product: [SW|ABC transporter] (ATP-binding protein)

essential: no

synonyms: yxkM

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.3|Regulatory ABC transporters]
• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.1|Terminal oxidases]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,975,088 3,976,791

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

[SW|Domains]

• has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
• [SW|ABC transmembrane type-1 domain] (aa 15-298) (according to UniProt)
• [SW|ABC transporter domain] (aa 321-561) (according to UniProt)

Structure

• [PDB|2HYD] (from Staphylococcus aureus, 27% identity) [pubmed|16943773]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|C0459C0D45E31561D281E016D86CD80DCAF13357|cydA]-[gene|E66EAB3C172ED8D1D26C675A7338B8153FB66987|cydB]-[gene|A65576E0261F1950A0DAD72B2432D97AB3EB2AA5|cydC]-[gene|244BF9E397FDB0691FFAA8C9D419B0AE417CACE1|cydD]
  description: [Pubmed|16207915]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [PubMed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex]: repression, [Pubmed|15231791], in [regulon|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex regulon]
  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|17322317], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|17322317]

Biological materials

Mutant

• MGNA-B747 (cydC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1746 NBRP B. subtilis, Japan]
• BKE38740 ([gene|A65576E0261F1950A0DAD72B2432D97AB3EB2AA5|cydC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATATCGAAACAGGTCTTTTC, downstream forward: _UP4_AAGGCACAACTGGGGGAAAG
• BKK38740 ([gene|A65576E0261F1950A0DAD72B2432D97AB3EB2AA5|cydC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATATCGAAACAGGTCTTTTC, downstream forward: _UP4_AAGGCACAACTGGGGGAAAG

References

• 16207915,17322317,10092453,15231791,9852001,24958725,15231791,17322317,16943773