nagZ

N-acetylglucosaminidase

Locus: BSU_01660

Isoelectric point: 9.76

Molecular weight: 70.40 kDa

Protein length: 642 aa

Gene length: 1929 bp

Function: cell wall recycling

Product: N-acetylglucosaminidase

Essential: no

E.C.: 3.2.1.52

Synonyms: yzbA,ybbD

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Utilization of cell wall components

Gene

Coordinates: 186,452 188,380

Phenotypes of a mutant

• increased autolysis PubMed

The protein

Catalyzed reaction/ biological activity

• cleaves muropeptides derived from peptidoglycan, but not peptidoglycan itself PubMed
• Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides (according to UniProt)

Protein family

• glycosyl hydrolase 3 family (single member, according to UniProt)

Structure

• 3BMX PubMed

Localization

• secreted (with signal peptide), remains to some extent cell wall-associated PubMed

Additional information

• The gene is mis-annotated in KEGG as an ortholog of beta-N-acetylhexosaminidase EC 3.2.1.52. It is marked in MetaCyc as similar to beta-hexosaminidase. No EC annotation is available in Swiss-ProtSwiss-Prot.supporting the annotation is available. PubMed

Expression and Regulation

Operons

• Genes: murQ-murR-murP-amiE-nagZ-ybbC
  Description: PubMed

  Regulatory mechanism

  • MurR: repression, PubMed, in MurR regulon

  Regulation

  • expressed in late exponential and early stationary phase PubMed

Biological materials

Mutant

• available in Christoph Mayer's lab PubMed
• BKE01660 (nagZ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGAAACTTCCTCCTATC, downstream forward: _UP4_AGACCGCTTTAATAAGGAGG
• BKK01660 (nagZ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGAAACTTCCTCCTATC, downstream forward: _UP4_AGACCGCTTTAATAAGGAGG

References

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