pyrP

uracil permease

locus: BSU_15480

pI: 9.50

mw: 45.41 kDa

protein length: 435 aa

gene length: 1308 bp

function: uracil transport in/out via proton symport

product: uracil permease

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.6|Nucleotide/ nucleoside transporter]
• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,619,023 1,620,330

The protein

Protein family

• [SW|xanthine/uracil permease family] (according to UniProt)
• [SW|Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily] (according to UniProt)

Structure

• [PDB|3QE7] (E. coli uracil transporter, 43% identity) [pubmed|21423164]

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]
  description: [Pubmed|8206849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1709162], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|PyrR]: termination/ antitermination, via [SW|RNA switch], in [regulon|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|PyrR regulon]

  regulation

  • induced in the absence of uridine nucleotides ([protein|search|PyrR]) [Pubmed|8206849]

Biological materials

Mutant

• BKE15480 ([gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGATTTCCCCCTGAT, downstream forward: _UP4_ACATCTGAACAACATCATAT
• BKK15480 ([gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGATTTCCCCCTGAT, downstream forward: _UP4_ACATCTGAACAACATCATAT

References

• 7868607,8206849,12896995,16133632,1709162,17322189,18763711,21423164