bcrC [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

undecaprenyl pyrophosphate phosphatase, produces the carrier lipid for cell wall synthesis, bacitracin resistance

locus: BSU36530

pI: 9.00

mw: 21.00 kDa

function: cell wall synthesis, resistance to bacitracin and oxidative stress

product: undecaprenyl pyrophosphate phosphatase

essential: no

ec: 3.6.1.27

synonyms: ywoA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.8|Biosynthesis of the carrier lipid undecaprenylphosphate]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.5|Biosynthesis of the carrier lipid undecaprenylphosphate]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|cell wall synthesis], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|heat shock proteins], [SW|resistance against toxins/ antibiotics], [SW|membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|SigI regulon], [SW|SigM regulon], [SW|SigW regulon], [SW|SigX regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,758,547 -> 3,759,128

Phenotypes of a mutant

• increased susceptibility to rare earth elements [Pubmed|22904278]
• a ''[SW|bcrC]'' ''[SW|uppP]'' double mutant is not viable (unless ''[SW|yodM]'' is artificially expressed) [Pubmed|27528508]

The protein

Catalyzed reaction/ biological activity

• undecaprenyl pyrophosphate --> undecaprenyl phosphate

Protein family

• bcrC/ybjG/ywoA family (according to Swiss-Prot)

Effectors of protein activity

• inhibited by heptaprenyl pyrophosphate which accumulates in a ''[protein|search|ytpB]'' mutant [Pubmed|24806199]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Additional information

• there is a second undecaprenyl pyrophosphate phosphatase in ''B. subtilis'', [protein|search|YubB]. However, [protein|search|YubB] seems to play only a minor role. [Pubmed|22904278]

Expression and Regulation

Operon

• ''bcrC

[SW|Sigma factor]

• [protein|search|SigM] [Pubmed|17434969], [protein|search|SigI] [Pubmed|18156261], [protein|search|SigW] [Pubmed|11866510], [protein|search|SigX] [Pubmed|12399481]

Regulation

• expression activated by glucose (3.3 fold) [Pubmed|12850135]
• induced by heat shock ([protein|search|SigI]) [Pubmed|18156261]
• induced by bacitracin
• induced by oxidative stress
• induced in the stationary phase due to the internal production of toxic peptides ([SW|SdpC], [SW|SkfA]) [Pubmed|26364265]
• expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]

Biological materials

Mutant

• MGNA-A214 (ywoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/214 NBRP B. subtilis, Japan]
• BKE36530 ''bcrC''::''erm trpC2'' is available from the Bacillus Genetic Stock Center

References

Reviews

• 27344142

Original publications

• 17434969,18156261,22904278,14612242,12486040,12399481,17434969,15838020,14651641,24068241,24806199,21926231,15946938,26364265,27528508,27578312