bcrC [2017-05-24 16:10:52]

undecaprenyl pyrophosphate phosphatase, produces the carrier lipid for cell wall synthesis, secondary bacitracin resistance determinant

locus: BSU36530

pI: 9.45

mw: 21.58 kDa

protein length: 193 aa

gene length: 579 bp

function: cell wall synthesis, resistance to bacitracin and oxidative stress

product: undecaprenyl pyrophosphate phosphatase

essential: no

ec: 3.6.1.27

synonyms: ywoA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.8|Biosynthesis of the carrier lipid undecaprenylphosphate]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.5|Biosynthesis of the carrier lipid undecaprenylphosphate]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,758,547 → 3,759,128

Phenotypes of a mutant

• 5-fold increased sensitivity to bacitracin [Pubmed|26815905]
• reduced growth rate [Pubmed|26815905]
• increased susceptibility to rare earth elements [Pubmed|22904278]
• a ''[gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]'' ''[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]'' double mutant is not viable (unless ''[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]'' is artificially expressed) [Pubmed|27528508]

The protein

Catalyzed reaction/ biological activity

• undecaprenyl pyrophosphate -→ undecaprenyl phosphate

Protein family

• bcrC/ybjG/ywoA family (according to Swiss-Prot)

Effectors of protein activity

• inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Additional information

• there is a second undecaprenyl pyrophosphate phosphatase in ''B. subtilis'', [protein|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|UppP]. However, [protein|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|UppP] seems to play only a minor role. [Pubmed|22904278]

Expression and Regulation

Operons

• genes: [gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|17434969], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [Pubmed|18156261], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|11866510], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|12399481], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]
  • [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]: sigma factor, in [regulon|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV regulon]

  regulation

  • expression activated by glucose (3.3 fold) [Pubmed|12850135]
  • expression is induced by bacitracin [pubmed|32019833]

Biological materials

Mutant

• MGNA-A214 (ywoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/214 NBRP B. subtilis, Japan]
• BKE36530 ''bcrC''::''erm trpC2'' is available from the Bacillus Genetic Stock Center

References

Reviews

• 27344142

Original publications

• 17434969,18156261,22904278,14612242,12486040,12399481,17434969,15838020,14651641,24068241,24806199,21926231,15946938,26364265,27528508,27578312,26815905