bcd [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase

locus: BSU24080

pI: 4.94

mw: 39.00 kDa

function: utilization of branched-chain keto acids

product: valine dehydrogenase, isoleucine dehydrogenase,
L-leucine dehydrogenase

essential: no

ec: 1.4.1.9

synonyms: bkd, yqiT

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|BkdR regulon], [SW|CodY regulon], [SW|SigL regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,502,659 -> 2,503,753

The protein

Catalyzed reaction/ biological activity

• L-leucine + H₂O + NAD⁺ = 4-methyl-2-oxopentanoate + NH₃ + NADH (according to Swiss-Prot)

Protein family

• Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)

Structure

• [PDB|1LEH] (from '' Lysinibacillus sphaericus'', 71% identity, 82% similarity) [Pubmed|8591046]

Expression and Regulation

Operon

• ''[protein|search|ptb]-[protein|search|bcd]-[protein|search|buk]-[protein|search|lpdV]-[SW|bkdAA]-[SW|bkdAB]-[protein|search|bkdB]'' [Pubmed|12823818]

[SW|Sigma factor]

• [protein|search|SigL] [Pubmed|10094682]

Regulation

• induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]

Regulatory mechanism

• [protein|search|CodY]: indirect regulation [Pubmed|24843172]
• [protein|search|BkdR]: transcription activation [Pubmed|10094682]

Biological materials

Mutant

• a ''bcd'' mutant and a ''[protein|search|bcd] [protein|search|ybgE] [protein|search|ywaA]'' triple mutant are available in [SW|Linc Sonenshein]'s lab

References

Reviews

• 7705101

Original publications

• 12427936,15241682,10094682,12823818,24843172