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thrR [Mon Jun 06 2016 13:10:44 GMT+0200 (CEST)]
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thrR [Mon Jun 06 2016 13:10:44 GMT+0200 (CEST)]

transcription repressor of threonine biosynthetic gene
Locus
BSU27910
Isoelectric point
6.00
Molecular weight
16.00 kDa
Function
control of threonine biosynthesis
Product
transcription repressor of threonine biosynthetic gene
Essential
no
Synonyms
pheB,yszB

Genomic Context

      
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Categories containing this gene/protein

Categories containing this gene/protein

Gene

Coordinates on the chromosome (coding sequence)
2,852,157 -> 2,852,600

The protein

Catalyzed reaction/ biological activity

  • control of the threonine biosynthetic genes (hom-thrC-thrB and thrD) PubMed
  • Protein family

  • UPF0735 family (according to Swiss-Prot)
  • Domains

  • putative DNA binding signature (aa 19-51)
  • ACT (aspartate kinase-chorismate mutase-tyrA) domain (aa 70-145)
  • Effectors of protein activity

  • lysine and cysteine (indirectly) inhibit DNA-binding activity of ThrR PubMed
  • Expression and Regulation

    Biological materials

    Mutant

  • BKE27910 (thrR::erm, available in the BGSC and in Jörg Stülke's and Fabian Commichau's labs) PubMed
  • Expression vector

  • pGP323 (N-terminal Strep-tag, in pGP172) (available in Fabian Commichau's lab) PubMed
  • pBP620 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Fabian Commichau's lab) PubMed
  • pBP622 (C-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP382) (available in Fabian Commichau's lab) PubMed
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab PubMed
  • References

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