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yugS [2019-09-06 08:24:22]
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yugS [2019-09-06 08:24:22]

spore membrane protein, similar to magnesium exporter
locus
BSU_31300
pI
5.13
mw
49.33 kDa
protein length
429 aa Sequence Blast
gene length
1290 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,216,163 3,217,452

    The protein

    Protein family

  • [SW|UPF0053 family](according to UniProt)

    • Paralogous protein(s)

  • [protein|B2E9D026B668C8530C406353E3400059388566FB|YhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|MpfA], [protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|YqhB], [protein|45F03AB13292BBF0554785C7C02FE29033EDD742|YrkA]

    • [SW|Domains]

  • [SW|DUF21 domain] (4-201)
  • 2 [SW|CBS domain]s (aa 220-281, aa 284-341) (according to UniProt)

    • Structure

  • [PDB|4HG0] (CorC from E. coli, corresponds to the [SW|CBS domain]s and the C-terminal transporter-associated domain)

    • [SW|Localization]

  • cell membrane [pubmed|30602489]

    • Expression and Regulation

    Operons

    genes
    [gene|A459410312A926365B45CEB4694DE399B38820A2|yugS]
    description
    [Pubmed|9274030]
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    Biological materials

    Mutant

  • MGNA-A632 (yugS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/632 NBRP B. subtilis, Japan]
  • BKE31300 ([gene|A459410312A926365B45CEB4694DE399B38820A2|yugS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAAGCCCTTACCATA, downstream forward: _UP4_TGATAAAAAACAGCCGGGGA
  • BKK31300 ([gene|A459410312A926365B45CEB4694DE399B38820A2|yugS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAAGCCCTTACCATA, downstream forward: _UP4_TGATAAAAAACAGCCGGGGA

    • Expression vectors

  • pGP2936 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 9274030,27933050,30602489,31415562