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recA [Thu Aug 25 2016 18:08:19 GMT+0200 (CEST)]
multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage), required to internalize and to recombine ssDNA with homologous resident duplex, required for efficient survival and replication restart after replication-transcription conflicts
Molecular weight
37.00 kDa
Function
DNA repair/ recombination
Product
multifunctional protein involved in homologous
recombination and DNA repair (LexA-autocleavage)
Genomic Context
Categories containing this gene/protein
Categories containing this gene/protein
This gene is a member of the following regulons
Gene
Coordinates on the chromosome (coding sequence)
1,764,645 -> 1,765,691
Phenotypes of a mutant
slower growth PubMeddrastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMedreduced sporulation efficiency PubMedstrongly reduced chromosomal transformation rate PubMed The protein
Catalyzed reaction/ biological activity
RecA stimulates ssDNA phosphorylase activity of PnpA PubMedRecA-ATP in concert with DprA and SsbA catalyzes DNA strand exchange, with SsbB as an accessory factor PubMedRecA-dATP catalyzes strand exchange even in the absence of the accessory factors PubMedprotects sporulating cells from DNA damage PubMed Protein family
recA family (according to Swiss-Prot)Modification
Effectors of protein activity
Structure
1U94 (RecA from E. coli, 62% identity, 86% similarity) Localization
colocalizes to the replisome in response to endogenous and exogenous DNA damage and in response to damage-independent fork arrest (formation of DNA repair centers), repair center formation depends on RecO and RecR, and is facilitated by RecF and SsbA PubMedNucleoid (Mid-cell) PubMedlocalizes to one cell pole PubMedco-localizes with the DNA uptake machinery PubMedforms a transient, mobile focus associated with the chromosome during spore development PubMed Interactions
Expression and Regulation
Operon
Sigma factor
Regulation
induced by DNA damage (LexA) PubMedinduced by conditions that trigger development of genetic competence (ComK) PubMed Regulatory mechanism
Additional information
Biological materials
Mutant
Expression vector
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab Labs working on this gene/protein
References
Reviews
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Original publications
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