yrhE

putative formate dehydrogenase

Locus: BSU_27220

Isoelectric point: 5.16

Molecular weight: 108.57 kDa

Protein length: 980 aa

Gene length: 2943 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 1.2.1.2

Synonyms

Genomic Context

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Categories containing this gene/protein

• Groups of genes → Proteins of unknown function

Gene

Coordinates: 2,781,209 2,784,151

The protein

Catalyzed reaction/ biological activity

• formate + NAD⁺ --> CO₂ + NADH (according to UniProt)

Protein family

• C-terminal part: prokaryotic molybdopterin-containing oxidoreductase family (according to UniProt)

Paralogous protein(s)

• YjgC

Domains

• 2Fe-2S ferredoxin-type domain (aa 5-81) (according to UniProt)
• 4Fe-4S His(Cys)3-ligated-type domain (aa 81-121) (according to UniProt)
• 2 4Fe-4S ferredoxin-type domains (aa 144-171, aa 187-216) (according to UniProt)
• 4Fe-4S Mo/W bis-MGD-type domain (aa 263-319) (according to UniProt)

Modification

• Fe-S cluster PubMed

Cofactors

• contains an iron-sulfur cluster

Structure

• 2FUG (from Thermus thermophilus, 23% identity) PubMed

Additional information

• The gene is annotated in KEGG as an ortholog of formate dehydrogenase EC 1.2.1.2. In Swiss-Prot the protein is named formate dehydrogenase chain A. In MetaCyc the protein is characterized as similar to formate dehydrogenase. No literature/experimental evidence supporting the annotation is available. PubMed

Expression and Regulation

Operons

• Genes: yrhE-yrhD
  Description: PubMed

  Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of yrhE PubMed

Biological materials

Mutant

• MGNA-A160 (yrhE::erm), available at the NBRP B. subtilis, Japan
• BKE27220 (yrhE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCATATTCCCCTCCA, downstream forward: _UP4_GATTAAACGATAGGAGGAAA
• BKK27220 (yrhE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCATATTCCCCTCCA, downstream forward: _UP4_GATTAAACGATAGGAGGAAA

References

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